DNA Data Requirements
17. What DNA data is accepted at NDIS?
Currently, DNA data generated through PCR Short Tandem Repeat (STR) technology, Y chromosome STR (Y STR) technology, and Mitochondrial DNA (mtDNA) technology are accepted at NDIS.
Y STR and mtDNA data are only searched with the missing person-related indexes.
The National DNA Index no longer searches DNA data developed using restriction fragment length polymorphism (RFLP) technology.
18. Are there specific data requirements for the DNA records submitted to NDIS?
Yes. There are several requirements for the DNA data submitted to NDIS:
- The DNA data must be generated in accordance with the FBI Director’s Quality Assurance Standards;
- The DNA data must be generated by a laboratory that is accredited by an approved accrediting agency;
- The DNA data must be generated by a laboratory that undergoes an external audit every two years to demonstrate compliance with the FBI Director’s Quality Assurance Standards;
- The DNA data must be one of the categories of data acceptable at NDIS, such as convicted offender, arrestee, detainee, legal, forensic (casework), unidentified human remains, missing person, or a relative of missing person;
- The DNA data must meet minimum CODIS Core Loci requirements for the specimen category;
- The DNA PCR data must be generated using PCR accepted kits; and
- Participating laboratories must have and follow expungement procedures in accordance with federal law.
19. What are the CODIS core loci?
Effective January 1, 2017, the CODIS Core Loci include the following 20 loci:
- CSF1PO
- FGA
- THO1
- TPOX
- VWA
- D3S1358
- D5S818
- D7S820
- D8S1179
- D13S317
- D16S539
- D18S51
- D21S11
- D1S1656
- D2S441
- D2S1338
- D10S1248
- D12S391
- D19S433
- D22S1045
The Original CODIS Core Loci, required from October 1998 until December 31, 2016, included the following 13 loci:
- CSF1PO
- FGA
- THO1
- TPOX
- VWA
- D3S1358
- D5S818
- D7S820
- D8S1179
- D13S317
- D16S539
- D18S51
- D21S11
20. What are the minimum loci requirements for the STR DNA data submitted to NDIS?
The minimum CODIS Core Loci required for submission of DNA data to NDIS vary by specimen category. Generally, the CODIS Core Loci are required for submission of convicted offender, arrestee, detainee, and legal profiles. The CODIS Core Loci and Amelogenin are required for relatives of missing person profiles.
All CODIS Core Loci must be attempted for other specimen categories with the following limited exceptions:
- For forensic DNA profiles, all CODIS Core Loci must be attempted but at least 8 of the original CODIS Core Loci combined with a match rarity of at least one in ten million are required for submission to and searching at NDIS.
- For Missing Person and Unidentified Human Remains, all CODIS Core Loci must be attempted.
21. What are the requirements for submission of mtDNA data to NDIS?
Hypervariable region I (“HV1”; positions 16024-16365) and hypervariable region II (“HV2”; positions 73-340) are required for the submission of mtDNA data to NDIS.
