A new post from the DDP facebook page, I wonder which case is a recent solve
Q: What is the smallest amount of DNA that DNA Doe Project has had success with? How many cells?
A: The number of cells can only be estimated from the amount of DNA extract obtained (approximately 6 or more picograms per cell). And those amounts are reported differently depending on the quant system used. The lab we use nowadays reports amounts provided by an ALU-based assay.
Some of our smallest amounts have included:
• A recently solved case not yet announced: 19 picograms (estimating approx. 3 cells)
• Lime Lady: 30 picograms (approx. 5 cells) - identified
• Redondo Beach Jane Doe: 26 picograms (approx. 5 cells) - in research, with usable data
When we first started in 2017 the conventional wisdom was that we needed at least 20 nanograms to get usable data (a nanogram is 1,000 picograms.) With the technological advances nowadays most labs experienced in whole genome sequencing now routinely produce usable data with fractions of a nanogram (well below 500 picograms).
We’ve come a long way!
Q: What is the smallest amount of DNA that DNA Doe Project has had success with? How many cells?
A: The number of cells can only be estimated from the amount of DNA extract obtained (approximately 6 or more picograms per cell). And those amounts are reported differently depending on the quant system used. The lab we use nowadays reports amounts provided by an ALU-based assay.
Some of our smallest amounts have included:
• A recently solved case not yet announced: 19 picograms (estimating approx. 3 cells)
• Lime Lady: 30 picograms (approx. 5 cells) - identified
• Redondo Beach Jane Doe: 26 picograms (approx. 5 cells) - in research, with usable data
When we first started in 2017 the conventional wisdom was that we needed at least 20 nanograms to get usable data (a nanogram is 1,000 picograms.) With the technological advances nowadays most labs experienced in whole genome sequencing now routinely produce usable data with fractions of a nanogram (well below 500 picograms).
We’ve come a long way!