https://www.tandfonline.com/doi/full/10.1080/20961790.2019.1642567
2019 rbbm.
''Semen stain is one of the most important biological evidence at sexual crime scenes. Age estimation of human semen stains plays an important role in forensic work, and it is rarely studied due to lack of well-established methods. In this study, the technique called attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR) coupled with advanced chemometric methods was employed to determine the age of semen stains on three different substrates: glass slides, tissues and fabric made of regenerated cellulose fibres up to 6 d.''
''Conclusion
This study demonstrates that
FTIR, combined with chemometrics, provides an efficient method for estimating the age of human semen stains based on time-dependent changes in the spectra.''