I dont remember where, but when I first posted the photo showing the red-stained area I noted that I couldnt verify its authenticity considering that it came from one of the tabloids. I dont know if they photoshopped it to indicate what they were told about the urine found on the carpet, or if it was an actual CSI photo showing the results of forensic testing. Jammie was indeed the first person who raised the issue, so for years there was always doubt about its credibility. I wouldnt even consider contradicting something
Cynic cites from his research, but the post you cited was based on what was known in 2010. My memory fails me on when and where, but sometime after that the existence of a urine stain was confirmed from a more reliable source than Lou Smits toadie. It was also confirmed by James Kolar in his AMA (Ill give you the quote below).
As for the red color shown in the questioned photo, I cant say with certainty what tests were or were not done by investigators. We all know that luminol is used for detecting residual blood. But because of its process, it wouldnt show urine. It is also something that only shows the presence of blood under fluorescent light sources, which the questioned photo was obviously not taken with. Thats the main reason I doubted the validity of this photo when I posted it.
However since you want more information, I did a little more research.
Nothing detects urine -- just like luminol doesnt really detect blood. Each of the tests detects elements found within the fluids. Luminol reacts with the iron in hemoglobin causing it to fluoresce. Besides luminol,
fluorescein is also used to locate possible blood; and
DMAC (dimethylacetamide) and
picric acid are use to located possible urine. Tests for urine look to identify
urea and/or
creatinine within the urine. From the book
Forensic Science (link below):
Creatinine is detected by applying a saturated solution of picric acid in toluene or benzene to a stain extract. It combines to form creatinine picrate, an easily detectable colored product.
From
Reactions of Creatinine with Alkaline Picrate:
...addition of 2 to 3 moles of alkaline picrate per mole of creatinine in aqueous solution resulted in the formation of a red compound. Color development, as in the Jaffe reaction, was essentially complete within 10 minutes at room temperature. The colored compound could be removed from solution by pouring the mixture into ethyl alcohol. Addition of acid to the precipitate permitted quantitative recovery of unchanged picric acid. If the aqueous mixture of creatinine and picrate stood for 30 or more minutes, some fading was noted, and subsequent recovery of picric acid was no longer quantitative.
Again, I still dont know what tests were done in the basement by investigators, and I dont know how accurate the questioned photo was. But apparently there are tests for urine that would show up as red. It seems like Ive seen a photo of the WC entrance after the carpet had been removed, but I cant locate it now.
Here is what James Kolar said in his AMA when asked about where the events occurred (emphasis mine):
Obviously, there is speculation as to where in the home that blow was rendered. No one, except for the person who struck her, knows for certain. I have my own theory, based upon the nexus of evidence present in the case, but as indicated previously, cant expound upon that at the moment.
I believe the commonly held theory, based upon the sequencing of injuries, is that the garroting and the location of her death was on the small piece of carpet located outside the wine cellar door. Her bladder let go upon death, accounting for the urine stains in the front of her clothing and carpet. The rear application of the garrote is confirming evidence of her being on her stomach during this act.
(Kolar may have also been asked about this in one of his interviews with Tricia. Im not sure.)
:findinglink:
http://science.howstuffworks.com/luminol.htm/printable
https://books.google.com/books?id=K...ad=0#v=onepage&q=urine identification&f=false (Begins on Page 272)
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http://www.jbc.org/content/237/2/612.full.pdf[/FONT]